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We next asked whether the spacing between the two core binding sites was important for FOXP3 DNA binding.
The H19DMD-ΔR mutation lacks the four core binding sites of CTCF in the DMD region.
The yellow labelled sequences show the short but conserved core binding sites within the promotor region.
Although the KMN is thought to provide the core binding sites for MTs at the KT, several other protein complexes also function at this critical interphase.
To exclude the possibility that the promoter structure might be affected by motif deletion, we also replaced the 6-bp Cbf1 core binding sites with a randomized sequence.
To address the specificity of NBS1 and NBS4 core binding sites we have introduced transversion point mutations to the core cognate elements aiming to disrupt the NKX3.1 homeodomain DNA recognition.
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IDEF1 recognizes the CATGC sequence within IDE1, whereas IDEF2 predominantly recognizes CA[A/C]G[T/C][T/C/A][T/C/A] within IDE2 as the core binding site.
IDEF1 recognizes the sequence CATGC within IDE1, whereas IDEF2 predominantly recognizes CA[A/C]G[T/C][T/C/A][T/C/A] within IDE2 as its core binding site.
Based on the crystal structure of ADT in complex with an oligopeptide segment corresponding to the core binding site of LHB, a sequence-specific amino acid preference profile was determined systematically for the ADT-binding peptides using structural bioinformatics approach.
Only binding site matches with >90% identity to the core binding site motif are reported here.
Only genes containing both a potential core binding site and a potential half binding site were considered as positive for the presence of a "complete QRE".
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