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CD59 transcripts in an adult gecko spinal cord were detected by preparing four segments along the anterior-posterior (AP) axis (i.e. cervico, thoracic, lumbar and sacral segment, refer to Material and Methods), and were normalized to endogenous EF-1α.
No abnormalities of the cervical spinal cord were detected on MRI.
Levels of expression of protein kinase C gamma (PKCγ) and NMDA receptor subtype NR1 in spinal cord were detected by Western blotting.
To validate the sensitivity and specificity of this method, NIR-labeled MSCs-IFN β in the brain and spinal cord were detected by immunofluorescence staining using an anti-hNA (human nuclear antigen) polyclonal antibody (Molecular Probes, Eugene, OR), followed by detection with an anti-rabbit secondary antibody conjugated with Alexa 488 (Molecular Probes).
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If cords were detected in only one control well, MODS-DST was recorded as uninterpretable for technical analysis.
Apoptotic spinal cord neurons were detected by TUNEL and the immunostaining of the neuronal marker neurofilament protein (NFP) using an in situ cell death detection kit (TMR red; Roche Applied Science, Mannheim, Germany), according to the manufacturer's instructions.
In the spinal cord few, small sized neurons were detected, especially around the central canal (Fig. 3).
Antibodies to SARS virus were detected in cord blood and breast milk.
These changes correspond with the results of immunohistostaining in the spinal cord, where GluN2B-positive cells were detected.
It has been shown that, after women in the last trimester of pregnancy were given trivalent inactivated influenza virus vaccine, high IgG antibody titers to maternal vaccine antigens were detected in cord and infant serum [ 12].
These polyfunctional IFN-γ/IL-2 CD8+ T-cell immune responses were detected in the cord blood of EU neonates and in the peripheral blood of EU as well as HIV-1 infected infants and children (Figure 3B).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com