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In addition to the urine specimens, dried umbilical cord specimens from the cases (n=23) were used for further confirmation.
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In addition to urine specimens, dried umbilical cord specimens were obtained from 23 of the cases for further confirmation.
Histology: Spinal cord specimens were removed from the examined animals after completion of the imaging protocol and placed in formalin.
The spinal cord specimens were processed using a rabbit antibody against Iba-1 (1:1000; Wako, Osaka, Japan).
The spinal cord specimens were obtained and dehydrated in 30% sucrose.
Furious patient no. 8 did not have spinal cord specimens available.
Brain and spinal cord specimens were embedded in paraffin and sectioned for histochemical analysis.
Unlike meconium, universal collection of umbilical cord specimens at birth is feasible.
The exclusion criteria prevent inclusion of brain and spinal cord specimens of poor quality.> -wrap-foot> Amyotrophicophic lateral sclerosis; UNITE, Understanding Neurologic Injury and Traumatic Encephalopathy Consent for donation of brains and spinal cords is acquired from the decedent's legal next of kin or legally authorized representative (LAR).
Proteomic profiling of the effects of progressive neurodegeneration has included cerebrospinal fluid, cervical spinal cord specimens, lumbar spinal cord preparations and skeletal muscles [ 33– 40].
Spinal cord specimens were incubated with Alexa 488-conjugated goat anti-rabbit IgG (for Iba-1 immunoreactivity, 1 1000).
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