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The amount of total protein in the spinal cord samples was measured with the PCA protein assay (PIERCE, USA).
Frozen plasma from 210 cord samples was transferred on dry ice to the Centers for Disease Control and Prevention for laboratory analyses for the PBDEs and PBB-153.
The success of pyrosequencing in the umbilical cord samples was 100%, 97%, 99%, and 91% for Alu, LINE-1, p16, and p53, respectively.
The difference between serum anti-GAG antibody levels of controls and umbilical cord samples was significant (three-way ANOVA, F = 110.70; DF = 1.77; p < 0.001).
In the results (Table 2), the percentage detection of PFOS, PFOA, and PFOSA in the maternal and cord samples was 100% (30 of 30), 10% (3 of 30), and 0% (0 of 30), respectively.
The difference between serum anti-glycosaminoglycan (GAG) antibody levels of controls and umbilical cord samples was significant (three-way analysis of variance [ANOVA], F = 110.70; DF = 1.77; p < 0.001).
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Human post-mortem paraffin-embedded and frozen brain material, and frozen spinal cord samples were obtained from the Netherlands Brain Bank (NBB), Amsterdam.
FFPE brain and spinal cord samples were deparaffinized with xylene and DNA extracted in a manner similar to frozen samples after treatment with 6 mg Proteinase K for 3 days at 55°C.
Frozen cord samples were cryostat-sectioned (15 µm thick) in a sagittal plane at Kalderon's laboratory; the serially collected sections were thionin stained and examined by light microscopy.
Additionally, seven MS spinal cord samples were tested.
Rats were euthanized after the final behavioral test and spinal cord samples were harvested.
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