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Electron microscopy confirmed that the anti-aconitase antiserum used selectively labelled mitochondria in spinal cord samples of control and transgenic mice (Fig. 5).
We also quantified the amount of Δ7Smn from our SmnC-T-Neo and Smn2B-Neo genotypes by comparing them to Δ7Smn transcripts derived from spinal cord samples of SmnΔ7/WT mice [34].
In particular, a recent study showed alterations in global methylation and hydroxymethylation in postmortem spinal cord samples of SALS patients.
Spinal cord samples of 1 cm length centered at the injury site were dissected, post-fixed and embedded.
FluoroMyelin stained cryosections indicated a much higher number of globular bodies (degenerated myelin) in both rostral and caudal cord samples of Lesion group compared with Lesion+Matrix animals.
In addition, increased levels of XBP-1s, ATF4 and GRP58 have been observed in human postmortem spinal cord samples of sporadic ALS patients by Western blot analysis [ 37].
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HOF was responsible for collection of umbilical cord samples for analysis and identification of patients.
(D ) RNA-seq data of spinal cord sample for Taf9b locus in WT and Taf9b KO animals.
Dupuytren's contracture (DC) cord samples and small samples of phenotypically normal palmar fascia tissue (PF) were surgically explanted at the Hand and Upper Limb Centre at St Joseph's Health Care (SJHC), London, ON, Canada.
Detergent extraction of cultured cells was performed as described in the foregoing reports; however, for spinal cord samples, slight modifications of the protocol were followed to obtain higher levels of extracted protein.
In the results (Table 2), the percentage detection of PFOS, PFOA, and PFOSA in the maternal and cord samples was 100% (30 of 30), 10% (3 of 30), and 0% (0 of 30), respectively.
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