It was further observed, that oligodendrocytes fail to develop in spinal cord explants derived from mice lacking neuregulin [ 198], that erbB2 is required for the development of terminally differentiated spinal cord oligodendrocytes [ 141], and that erbB2 plays a role in governing the properly timed exit from the cell cycle during development into myelinating oligodendrocytes [ 89].
The central nervous system consists of the brain and spinal cord, both derived from the embryonic neural tube.
Our results demonstrated that ZIKV directly invaded and robustly replicated in the brain and spinal cord organoids derived from human pluripotent stem cells.
Perfusion of the distal spinal cord is derived from the lumbar, iliolumbar, and lateral sacral arteries.
Colocalization was assessed in a total of 321 and 323 terminals in spinal cord sections derived from MGL+/+ and MGL−/− mice, respectively (n = 3 3 animals).
Conversely, MGL-immunoreactive precipitate was not visible in spinal cord sections derived from MGL−/− mice, confirming the specificity of the staining (Fig. 1B).
The adult spinal cord is derived from the caudal neural tube, which is initially composed of heterogeneous neuroepithelial cells surrounding a central lumen with distinct proliferative and differentiation potential.
At the light microscopic level, a striking accumulation of dense MGL-positive immunostaining was detected in the dorsal horn of spinal cord sections derived from MGL+/+ mice (Fig. 1A).
To test whether Aptx deficiency exacerbates the motor neuron loss in SOD1G93A mice, we compared Nissl-stained motor neurons in spinal cord sections derived from wild-type, Aptx−/−, SOD1 G93andnd Aptx−/− SOD1 G93A mice (Fig. 4).
In line with our observations utilizing an immunoperoxidase approach, the nature of MGL-positive fluorescent immunostaining consisted of a dense, punctate labeling pattern throughout the superficial dorsal horn of spinal cord sections derived from MGL+/+ mice (Fig. 4A).
