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We imaged approximately 15 20 images per spinal cord cross section, depending on the size of the cord after injury.
For analysis of the area of spared tissue, images of whole spinal cord cross sections stained with GFAP were taken at 100× magnification and analyzed in ImageJ (NIH).
The total average number of positive cells per square millimeter, in spinal cord cross sections, was counted using a grid overlay.
There were 2% motoneurons with ischemic cell changes per spinal cord cross section in intact control).
The number of MNs per anterior horn (either left or right) of the spinal cord cross section was counted.
In addition, there were 1% motoneurons being identified as ghost neuron per spinal cord cross section in the intact controls).
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Spinal cord cross-sections were subsequently cut on a cryostat at 10 µm.
c, A schematic spinal cord cross-section showing progenitor and transcription factor domains. d, Double immunolabelling of Dmrt3 and Pax7 shows that Dmrt3+ cells originate from the ventral-most part (bracket) of the dorsal domain (border indicated by line).
Dynamic changes in the spinal cord cross-sectional area due to cervical ossification of the posterior longitudinal ligament (C-OPLL) are unknown, but dynamic multidetector-row computed tomography (MDCT) may be a useful tool.
The proportion of motoneurons exhibiting such inclusions varied from only a few to most of the motoneurons, and they were seen in all spinal cord cross-sections investigated (Table 1).
A minimum of 12 spinal cord cross-sections were examined per animal.
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