A 15-mm sample of spinal cord centered on the epicenter was harvested, weighted, and crushed to powder.
The laminectomy was extended, and a 20-mm-long fragment of spinal cord centered on the epicenter was harvested and post-fixed in 4% paraformaldehyde.
A 1 cm-length of the spinal cord centered at the injury site was removed, snap-frozen in liquid nitrogen and stored at −80°C until further processing.
Ten days after treatment, animals were killed and a 1 cm length of the spinal cord centered at the injury site was separated.
We evaluated the lesion area on a large segment (3-cm corresponding to 3 metamers) of the spinal cord centered on the lesion that included the 3 grafting points.
The histological sections of the liver from the control animals from each species demonstrated that the structural integrity of the hepatic lobules was intact, there was actinomorphous distribution of the hepatic cord centered by central veins, polygonal hepatocytes and no edema (Figure 1g, i, k).
For protein samples from the spinal cord, a 0.5 mm segment of the spinal cord, centering at the injury site, was dissected out from the sacrificed animal.
A 1.0-cm segment of spinal cord centered at the injury epicenter was sectioned at 20- μm thickness and thaw-mounted onto Superfrost Plus slides (Fisher Scientific, Pittsburgh, PA, USA).
Compared with the yellow cattle, the structural integrity of the hepatic lobules was intact in the livers of infected water buffalo, there was actinomorphous distribution of hepatic cord centered on central veins, polygonal hepatocytes, no edema, no inflammatory cell infiltrated, and only scattered neutrophils and monocytes.
Earlier in the day, LeGrand was transferred to a New Jersey spinal cord rehabilitation center.
