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This study provides evidence for the first time that ASC scaffold seeded with hUCB-MSCs is able to bridge a spinal cord cavity and promote long-distance axon regeneration and functional recovery in SCI rats.
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The resulting pulsatile flow and pressure wave propagation, and their possible effects on cord cavities and cord stresses, have been elucidated.
Our data suggest that the retracted axons in the ascending sensory tracts extended through the glial scar surrounding the lesion site distal to the hydrogel/microtube filled spinal cord cavity due to the delivery of Cdc42, Rac1, and BDNF.
Microtubes loaded with BDNF/Rhodamine embedded in the agarose were injected into the spinal cord cavity.
Lipid microtubes loaded with BDNF/Rhodamine embedded in the agarose were injected into the spinal cord cavity.
Our present results strongly suggest that when pHPMA hydrogel is implanted in lesioned spinal cord cavity, there might be a sensori-motor reorganization that allows the transmission of the sensory messages from the sub- to the supra-lesional spinal cord.
Previous studies showed that pHPMA hydrogel implanted into the transected or compressed spinal cord cavity promoted the formation of a replacement tissue with histotypic characteristics of the host (Woerly et al., 2001a, 2001b).
First of all, 8 × 10 autologous MSCs were directly injected into the intradural space, after 4 and 8 weeks, another 5 × 10 MSCs, each time, were injected into the spinal cord above the lesion cavity and into the cavity, respectively.
Another injured his spinal cord; others "perforated" his chest cavity and lungs, he said.
Dose-volume histograms of the PTV and organs at risk (OARs), such as auditory system, oral cavity, and spinal cord, were compared.
For the brain stem, spinal cord, larynx, parotid glands, oral cavity, and skin, RA-FFF delivered a significantly increased dose exposure compared to RA-C.
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