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For each treatment and each time point, TUNEL-labelled nuclei were counted in coral sections obtained from 6 coral branches (6 sections were analysed and averaged per coral branch), using the same magnification (x40) and compared to the total number of host cells in the same image field to obtain a percentage of TUNEL-positive host cells.
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In the Coral Gables section of Miami, he said, "three contracts have fallen through in the last week; nothing's wrong with the house -- people kept getting cold feet".
To determine the integrity of coral tissue selected sections were counterstained with Haematoxylin & Eosin following routine histological procedures.
Over time, Edward placed the carvings on his new property in Homestead, excavated additional gigantic sections of coral, and erected – literally – a castle, complete with lookout walls and a forlorn but breezy turret in which he slept.
"The new video and stills are very concerning and show large sections of coral drained of all color and fighting for survival," WWF spokesperson Richard Leck said in a statement. .
The study, published in the journal Science Advances, has identified the unique sections of coral DNA that can indicate a higher tolerance to environmental stress factors like unusually warmer water, excess sunlight, poor water quality, and ocean acidification.
In September 2013, the state government's environmental agency fined the port and industrial complex 2.5m reals for causing environmental damage, exemplified by the dynamiting of a section of coral reef near the port.
The reef is in the middle of the worst bleaching event ever seen, with unusually warm water killing as much as half the corals in the northern sections, with the trend set to continue for the next 20 years.
In addition to Byrd, the boat was also loaded with at least 50 years of experience in Maui diving, much of which belonged to Dan Saunders, a marine archaeologist with Robinson's who could rattle off about every species of fish and coral that inhabited this section of reef.
We used data from Halpern et al. [6] that depicts the impact of anthropogenic drivers of change (henceforth referred to as threats), to each 1 km2 section of coral reefs [6], [20].
As a result it was possible to accurately measure Asx D/L of the intra-crystalline Free fraction, and demonstrate for the first time the sensitivity of this measurement as a dating tool in coral (Fig. 6b; Section 4.3).
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