Exact(18)
The results indicate that integration of the gene cassette into multiple copy loci should be given consideration when designing amylolytic yeast strains.
The infant is likely to have had disseminated primary tuberculosis: - the only DNA sequences for single copy loci were obtained from the infant material, which suggests a higher bacterial load during life.
Single copy loci showing heterogeneous hypomethylation comprise SCP-1 [12], the imprinted loci D15S9, D15S63 and H19 [3] and in female ICF cells a number of genes residing on the inactive X chromosome [3], [12], [13].
In effect, this means that single copy loci were favoured over those with multiple copies.
Single copy loci were identified as the class of 100 bp fragments with only one match.
Single copy loci reference gene measurements were also compared with the multicopy genomic repeat ALUJ.
Similar(42)
Several methods have been developed to differentiate genotypes currently dominated by microsatellite markers targeting single-copy loci.
The Y-STRs comprised of 8 core loci, 2 multi-copy loci and other 14 Y-STR loci.
The Quantifiler HP and Trio Kits use multiple-copy loci to allow for significantly improved sensitivity compared to earlier-generation kits that employ single-copy target loci.
Amplification of aDNA, using specific PCR primers designed for short fragments and linked to fluorescent probes, gives good results, especially when designed to target multi-copy loci.
Overall, this method provides an efficient means of isolating predominantly single-copy loci from both low and high-copy gene families, providing ample nuclear variation for reconstructing species-level phylogenies in non-model taxa.
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