The 5′ extension of SINEU-2B_Crp shows weak similarity to nucleotides 275-231 of Mariner-N4_AMi, indicating that the master copy for these SINEU-2B_Crp insertions is a SINEU-2B_Crp copy inserted into a Mariner-N4_AMi copy.
After electroporation of the resulting vector, pPICZα-A-Hepc, into the yeast host strain GS115, transformants with high copy inserts were selected by 1500 mg/L Zeocin™ selection.
It is an LT2 strain containing an nrdA'::ΩSpc'nrdB mutation and an extra nrdEF copy inserted elsewhere in the chromosome (merodiploid) allowing aerobic growth.
In one example, a copy inserted itself into a TTAA sequence of a sequence containing the S. salar MHC class 1b locus, a second copy inserted itself at a TTAA sequence inside the first copy, and a third copy inserted itself into the second copy.
Although MosSCI usually generates single copy inserts, the oxSi91 transgene was a rare event specifically selected for high expression and likely represent the insertion of multiple copies.
In the case of gene duplication, we distinguish between the gene that remains in the original location and the copy inserted into a new location.
In any case, a transposition event may generate a duplicated copy, inserted into a new genomic site, with a sequence that is identical to the original element.
Due to the LTR retrotransposon replication cycle, when a new copy inserts in the genome, its two LTRs are identical in sequence.
A significant increase in the efficiency of selection cassette recycling was demonstrated in the presence of single copy inserts when compared to multicopy concatemers (Fig 7C vs 5C).
Single copy inserts at the ttTi5605 locus were fully sequenced to confirm a lack of deletions or other mutations prior to use of transgenes for complementation tests.
The same infinitesimal (at the evolutionary scale) transition period exists when a functional copy inserts in a site where it brings some selective advantage, the copy being both potentially selfish and useful.
