Fosmids identified to contain polyubiquitin genes were grown in 50-ml cultures containing 50 μl of fosmid induction solution (Epicentre Biotechnologies) to increase copy number, as per manufacturer's protocol.
Initially, in the operational process, the AMSSDR selector will be utilised to select an appropriate resampling algorithm (for instance, systematic resampling technique or rounding copy resampling technique), as per the physical memory available in present computing devices.
To rescue per-function, we used transgenic flies carrying a wild-type copy of per (designated as perG) in a per background [ 35].
Recurrent somatic copy-number alterations in candidate regions identified by ENVE in the WES dataset were validated using a qBiomarker qPCR copy-number array as per the manufacturer's instructions (Qiagen Inc., Valencia, CA, USA).
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To verify that these effects are indeed linked to the lack of per gene function, we tested the lifespan of per flies transformed with a wild type copy of per, designated as per { per +}.
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Copy discarded as duplicate per D.G.W. 6/10/42. 1 map : col.
Since mRNAs are copied from genomic DNA, the simplest way to express mRNA level would be as copy number per template DNA, or more practically, as copy number per cell.
The copy number was then normalized to cell number and was displayed as copy number per cell.
Data were expressed as copy number per nanogram cDNA.
