We first immunoprecipitated from 6 fmol to 0.6 amol of plasmid (representing about 33,000 to ≈10 pcopiescopies), and finally by real-time PCR quantification (qPCR), 1 µg, 0.1 µg and 0.01 µg (0.278 pmol to 2.78 fmol) of DNA were immunoprecipitated in duplicate.
This progressive expansion can be seen from lemur (2 3 copies) to new and old world monkeys (30 35 copies) to apes (90 125 copies) and finally to human (272 copies in the hg19 build).
We calculated the dN, dS and K ratios (Table 3) between each O. minuta copy and its orthologs in the diploid genomes, between the paralogous and paleologous copies themselves and, finally, between the surrogate diploid progenitors.
Cahill flipped through a copy of Science News and, finally, an hour or so later, walked the basset hound to the road, picking him up for the dangerous crossing, then down four houses, where he saw that Breezy's car was gone and the back gate unlatched.
Copy and Paste might finally come to your iPhone.
Later, the program moved to sight word vocabulary, spelling pattern anomalies (e.g., "Wind the watch in the wind"), and other beginning reading matters, thereby establishing a progression from copy, to recognition, recall, and, finally, some of the many exceptions in English orthography.
Initiation, where RNA polymerase engages with the DNA template, followed by elongation where it translocates along the DNA template synthesizing the RNA copy of the gene and finally termination, where polymerase disengages from the DNA template.
Orchestra librarians scurried to find copies of the music in Beijing, and finally additional copies were flown up from Shanghai.
Using this simulation, patient-specific abutments were digitally designed, printed in 3-dimensions (3D), and finally copy-milled in titanium.
Today, this involves the disjointed tasks of message composition on a web-mail service, mapping the address on a map site, searching for reviews on the restaurant on a search engine, and finally copying all links into the message being composed.
