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To study the relationship between mating success and meat sharing we used GLMM [24] and set as the response variable the dyadic copulation frequency of each male-female dyad and as a predictor variable male-female meat sharing.
The original Wolbachia-infected wild-type strain was itself male-courtship defective, when tested in this assay with Oregon R females (giving a copulation frequency of only 57%).
DOI: http://dx.doi.org/10.7554/eLife.07643.007 10.7554/eLiFigure43.008 Figure 1 figure supplement 4. Courtship index and copulation frequency of control or mechanically sleep-deprived males (n = 15 control and deprived).
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To test this hypothesis, we collected data on meat sharing and copulation frequency in a group of wild chimpanzees in the Taï National Park, Côte d'Ivoire between 2003 and 2006.
The resulting equation of copulation frequency (copulation frequency (y) = intercept+estimate meat sharing*x: y = 1.55+1.56*x, see Table 1) indicates that if a male shared meat with a particular female, his mating success on average was twice as large (y = 1.55+1.56*1 = 3.11) as if he did not share meat with the female (y = 1.55+1.56*0 = 1.55).
Copulation frequency was calculated as percentage of flies in each condition that successfully copulated during the 10 min assay.
The copulation frequency correlated neither with the proportion of fights won by males nor with the frequency of male countermarking on female scents.
Copulation frequency was higher on the natural substrates of pine litter and red clay as compared with leaf litter.
We did this to test alternative explanations to having a relationship between meat sharing and copulation frequency and additionally to reduce, in each case, the number of predictor variables included in the model.
Copulation frequency assays were performed in single-pair assays in chambers of 1 cm diameter for 10 min under constant light.
For example, the ectopic expression of DmelOBP99b could reduce virgin female receptivity and copulation frequency [ 45].
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