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The Mann–Whitney U-test was used to determine the significance of differences in percent circling and percent copulation between the crosses.
Previously, Suotoni et al. (2006) reported no mate recognition (circling + copulation) between the well-defined Clade B phylotypes Cayman and Almenara and a 4-fold reduction in mate recognition between Almenara and B. ibericus.
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Furthermore, the species-level boundaries identified with mtCOI are supported by all available mating studies which show that the different members are mostly unable to copulate and where copulation occurs between the more closely related members of the complex, the resulting fitness of F1 and F2 progeny is considerably inferior to that of the parents [ 15, 16].
For 17 males that copulated at least once and masturbated during female oestrus, I calculated the percent of his masturbations that occurred before the first copulation, between copulations, and after the last copulation.
Crosses between different phylotypes have shown a gradient in mate recognition and copulation between members of the species complex [ 17, 22].
However, the proportion of sperm used for a single copulation differed between the two species (i.e., 8.4% in M. pruinosa and 32.5% in C. cornelia).
Finally, we found no relation between the time interval between successive copulations with different males and the likelihood of a female producing copulation calls (Wilcoxon exact test; Z = −0.314, Nsilent = 6, Nvocal = 6, p = 0.844).
At a recent Skutr happening at Fabrika, a factory turned 200-seat stage in the up-and-coming Holesovice district, the actors moved freely among the audience, in one instance pretending to find electric sex toys in people's pockets before turning them into musical instruments; in another, letting the audience's reaction set the pace of copulation between a butterfly and a fly.
We used a Wilcoxon rank sum test to determine if there were differences in the latency to copulation between conspecific and heterospecific crosses.
Extra-pair copulation (EPC), i. e. copulation between one individual and another of the opposite sex that is not its social partner, a pre-requisite for EPP, can be frequent in breeding colonies [ 9- 11].
Copulation between pairs seems to be closely related to food transfers, with this possibly serving as a trigger for intercourse.
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