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Romero, A. et al. X-ray analysis and spectroscopic characterization of M121Q azurin: A copper site model for stellacyanin.
This result confirms that the chemical modification with PEO2000 does not cause any structural change around the copper site.
Here we describe our recent efforts using EPL to incorporate nonproteinogenic cysteine and methionine analogues into the type 1 copper site found in Pseudomonas aeruginosa azurin.
The metal is usually found as a mononuclear type 1 copper site protected by a protein envelope, which has become known as a cupredoxin fold.
Our evolved laccase was tested in both human plasma and blood, displaying catalytic activity while retaining a high redox potential at the T1 copper site.
The SERS experiments in concert with resonance Raman effect confirmed that the structure at the "blue" copper site of the immobilized protein remained intact.
In this study, three separate mutations (M298F, V290N and V290A) were introduced at or near the T1 copper site of the small laccase (SLAC) from Streptomyces coelicolor A3(2) and biochemical properties were assessed in comparison with the native enzyme.
BOD, physically adsorbed on the mesoporous matrix of a CCG electrode, allowed a direct electron transfer (DET) from the carbon electrode to the type I copper site of the enzyme.
These results indicate that oxygen adsorption, NO decomposition and NO2 formation happen at the same type of copper site which is believed to be the copper center bonded with ELO.
DFT modeling of the adsorption of the acetone molecule on copper site gave the information about the function of the zeolite as a host for transition metal cationic sites and as an electron reservoir.
The results indicate that SO2 was catalytically oxidized to SO3 over a copper phase in the presence of gaseous oxygen, and then reacted with a copper site to form a sulfate linked to copper without desorption into the gas phase.
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