Sentence examples for copies was utilized from inspiring English sources
No cross-reactivity was observed when plasmid template representing 5 × 106 gene copies was tested, but it was evident (Ct values ≤30) in few instances when plasmid template representing 5 × 109 gene copies was utilized, the latter corresponding to the total gene copy number (as determined in this study) in 20 insects.
Positive controls (DNA extracted from M. smithii DSM861 and M. stadtmanae DSM 3091 strains, German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany), an extraction control, no-template controls, and a quantification scale from 10−7 to 10 target copies were utilized in each reaction plate.
In instances where the CXR was not available in digital format, de-identified hard copies were utilized.
Interestingly, of all 6 gene duplications included in our data sets (independent BRh duplications in Lycaenidae and Pieridae, two LWRh duplications in Papilio plus one LWRh duplication in Bombyx mori and one in Apodemia mormo), only one results in different groupings depending on which copy is utilized.
(2)ΔΔCq, where ΔΔCq= SMN1 Cq reference gene Cq average ΔCq of 2-copy calibrators, was utilized to estimate the SMN1 copy number.
In this large retrospective study we did not find evidence that copied documentation of lifestyle counseling was utilized to raise the level of E&M charges.
A machine learning approach was utilized to model DNA copy number amplifications in a landscape of cancers.
The quantitative PCR (qPCR) technique was utilized to validate the copy number variation of the ALK gene observed by FISH.
The CGDS-R package was utilized to explore putative copy number alterations of Notch receptor genes in the CCLE data set.
If the information above was insufficient for distinguishing the ancestral and the derived copies, the total synteny within 20 upstream and downstream gene loci was utilized.
The suicide mini-CTX2 plasmid based method was utilized for site-specific integration of the second copy of fabG.
