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For the ZFNs, the knock-out effects are at the level of the genomic DNA, and if total knock-out is not achieved in all of the copies, the expression of a functional mRNA will still take place, and thus the translation into the functional PAM protein will not be fully blocked.
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And I might say 'I may have taken the idea of having a fighter, but I didn't copy the expression that you made of that idea'.
Generally, third parties are allowed to use the ideas contained in the game so long as they don't copy the expression of those ideas from the original work — such as the underlying software game code or those creative elements of the game that make up the "look and feel" of the copyrighted work.
If there is some copying, the courts typically look to whether the allegedly infringing work is "based upon" or "substantially similar to" the original work, and third parties are allowed to use the "ideas" contained in the original work as long as they don't copy the "expression" of those ideas from the original work.
For each P. falciparum gene, its expression values in the 14 P. yoelii samples were extrapolated by copying the expression vector of its P. yoelii best ortholog match.
For example: In step2, The written expression was in I2, but I23 … copied the expression automatically.
The cell with the same structure will copy the expression automatically and compute.
The noncoding C9orf72 repeats, expanding from 700 to 1,600 copies, inhibit the expression of one alternatively spliced transcript, and induce the formation of nuclear RNA foci composed of the hexanucleotide repeat [ 1].
Insertion of Pfs25-IMX313 inthethe P. pastoris genome was done by homologous recombination of one or more copies of the expression plasmid into the TRP2 gene.
This could be a consequence of AcrA overexpression, caused by integration of multiple copies of the expression vector in the P. pallidum genome.
The analysis revealed that at least three copies of the expression vector consisting of FAD and FADO genes are present in the genome of the TDR transformant.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com