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The integration numbers of NTS-based expression cassettes, ranging from one to ∼30 copies, showed a proportional increase with the extent of decreased expression of the auxotrophic markers.
Copernicus's fame and book made its way across Europe over the next fifty years, and a second edition was brought out in 1566.[13] As Gingerich's census of the extant copies showed, the book was read and commented on by astronomers.
This could be seen in the cover art where a single image of a woman's profile was broken down within the repetition, and some of the copies showed the woman's teeth through her cheek.
Whereas no ISAba1 copy was identified on any of the plasmids of isolate AYE, chromosomal copies showed no site integration preference (Figure 3).
The results indicate that most tumors with one copy loss of PTEN had loss of PTEN expression (11/16, 69%) while three out of four cases with maintenance of two PTEN copies showed loss of PTEN expression.
For instance, males 3B and 10B, with 2 copies of the SRY gene, showed 1.5 2 times the level of expression, whereas 9F, 10F, 31F, 32F, 33F and 33B, also with two copies, showed higher levels (around 4 9 times) of expression.
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Another proposal is watermarking DVD's so that if copies show up online, their owners can be tracked.
Kirkus, known for its often-tart reviews, can heavily influence what books are bought for public libraries and how many copies show up in bookstores.
The apparent amplification of gene copies shown here suggests an inherent flexibility in the genome.
Surprisingly, each of the human DUXY2 4 gene copies show a unique nucleotide sequence at this codon position.
Chimpanzee DUXY2 4 gene copies show an identical stop codon (TGA) at codon 21 of the putative ORF, whereas all orthologous human copies do not show a stop codon at this position.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com