Sentence examples for copies recovered from inspiring English sources

We used a standard curve constructed from serial dilutions of pure plasmid pREP10 hGas67 as reference in qPCR experiments to determine the number of plasmid copies recovered from cultured cells and tissue samples after Hirt extraction.

The large variation in MBL gene copies recovered from control Guthrie cards indicates limitations in the DNA quality or the DNA extraction procedure.

To understand the duplication history of the multiple FAD2 and FAE1 copies recovered from C. sativa, we amplified the FAD2 and FAE1 genes from several Camelina species and outgroup species, and inferred phylogeny for each gene.

To better understand the C. sativa genome and to determine whether the multiple copies recovered are the result of polyploidization, we analyzed the genome sizes of C. sativa and its closest relatives in the genus Camelina by flow cytometry.

Examining the two miR-208 copies recovered in mammals, the paralogs miR-208a and miR-208b, we detected that both were intronic of Myh6 and Myh7, respectively, in agreement to a previous report in mouse and human [ 38].

However, pre-treatment of cells with HXR9, but not CXR9, causes a significant reduction in the number of HOXA2 promoter copies recovered using this technique, indicating that HXR9 does indeed directly disrupt the formation of a HOX/PBX complex.

Differential PCR recovery could occur because a given gene copy sustained mutations that make it a poor substrate for amplification relative to the copy recovered and/or is present in much lower copy number.

Assuming that the Sh ble gene is incorporated at the same ratio as the AOX1 TT sequence it generally seems like the presence of 1 copy of the Sh ble zeocin resistance gene is the minimum requirement for growth at 100 μg zeocin/mL, 4 copies at 500 μg/mL and 9 copies at 1000 μg/mL, but clones with as many as 17 gene copies were recovered from plates with the highest antibiotic concentration.

Oct4 RNA levels were not significantly different when calculated separately for female and male embryos in order to allow a better comparison to Xist measurements, which included only female blastomeres (49.4 ± 60.2 Oct4 DNA + RNA copies per recovered male cell vs 47.3 ± 41.2 Oct4 DNA + RNA copies per recovered female cell).

The mean Oct4 RNA content of our samples was lower than the mean Xist RNA content (48.7 ± 54.0 Oct4 DNA + RNA copies per recovered cell, average ± s.d. of 91 blastomeres, vs 75.1 ± 75.3 Xist DNA + RNA copies per recovered cell, average ± s.d. of 43 blastomeres).

Even F.B.I. and Interpol agents joined the chase; a few stolen copies were recovered.