As an example, the dispersion of 459 pg of sample DNA (containing 70 copies of a SNP allele from a heterozygous genotype) across the 765 chambers of a panel results in most chambers being unoccupied with target DNA sequence, but those 70 copies present are mostly isolated in individual reaction chambers.
Copy number of regions in the genomes of all the 16 individuals was detected by the read depth (RD) method [ 26, 34], where the number of copies present is inferred from sequence depth of whole genome sequence data.
Second, the R. typhi sequences (including duplicate copies if present) were selected for comparison with related proteins from other bacterial T4SSs.
Additional copies, when present, are very divergent and display nonconserved genomic contexts.
Theoretically, gene dosage quotients close to 1.0 indicate two copies present (that is, wild-type); 0.5, one copy absent (that is, hemizygous); 0.0, both copies absent (that is, homozygous deletion); and 1.5, one copy duplicated.
Our blast results suggest that very few NUPTS are in the nuclear genome (<3 kb), and the few copies that are present are highly degenerate.
Loci for which there was at most a single copy of a gene present were retained.
Quantification of the genomic copies present in the samples was less reliable (Additional file 1: Table S1).
The family with the second highest copy number (207 copies) is present in the fruit fly D. ananassae, and that with the highest copy number (397 copies) in the carolina anole Anolis carolinensis, an arboreal lizard.
Our evidence suggests that 11 nonhuman primate species have one RHOXF2 copy, and two copies are present in humans and four Old World monkey species, and at least 6 copies in chimpanzees.
