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Copies of total HIV-1 cDNA were calculated per 200 000 cells, the number of PHA-PBMCs in each sample.
To avoid this problem, we constructed a plasmid (4∼5 kb) containing a 300∼400 bp segment identical to the human genomic DNA region of interest; the copy number for the wild-type sequence can now be elevated to 1011 per µl along with 106 copies of total wild type genomic DNA.
We set the buffer size to different copies of total protein A and protein B combined that can be synthesized.
The risk of SLE was substantially greater in subjects with only two copies of total C4, but those with five or more copies of C4 had a reduced risk of disease.
First, total HIV-1 DNA was measured in gDNA samples by ddPCR and based on these measures, each sample was diluted to approximately five copies of total HIV-1 DNA per PCR replicate and distributed in replicates.
Absolute gene copy number was expressed as the number of copies of resistant genes per g−1, whereas relative copy number was expressed as the number of resistant genes per million copies of total bacteria ribosomal genes, as determined with the universal prokaryotic primers TBA-F and TBA-R (Table 1).
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I treasured my tattered copy of "Total Loss Farm," which sang to me of a mythical place where chosen families lived in a constant state of childlike wonder.
I didn't think any more about it until I picked up this month's copy of Total Film which had an A3 poster of the image inside.
A cDNA copy of total RNA was prepared using the SuperScript III reverse transcriptase first strand cDNA synthesis kit (Invitrogen, Carlsbad, CA) according to the manufacturers instructions, using oligo dT 15 (Roche Molecular Systems, Pleasanton, CA) to prime the reactions.
The 3-D aggregates were challenged at an MOI of 25 (≈4 × 10 copies of NV total) with live or γ-inactivated NV or mock-challenged with media alone.
We analyzed the distribution of clean tag copy number in the two libraries and found that the copy number of total clean tags and distinct clean tags showed very similar tendencies for two samples, respectively (Supplementary Material).
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