Because each A673 cell only has about 1000 expressed copies of fusion transcript (data not shown), 0.2 µg of A673 RNA contains approximately 3.3 × 10−5 fmol of EWS/FLI 7/6 fusion transcript.
However, the fusion to GP64 restricts the display to the poles of the virions as well as the number of copies of fusion proteins, and other strategies using fusion to VSV-G glycoprotein have therefore been proposed [ 16, 17, 20, 21].
However, even after adjusting for age and clinicopathological factors, there was some evidence that samples with multiple copies of the fusion conferred worse survival than those with no fusion or single copies of the gene fusion.
In addition, we found the rs12329760 SNP in TMPRSS2 to be significantly associated with fusion by translocation and with multiple copies of the fusion protein.
In its debut month of November 2002 in the United States, 199,723 copies of Metroid Fusion were sold, with total revenues of $5,590,768, making it the third bestselling Game Boy Advance game in that month, and the tenth bestselling game across all platforms.
The triplicated region of chromosome 4 contains two copies of a fusion gene comprising the N70 terminal amino acids of Ube4b and the entire coding region of Nmnat1 (C Terminal 285 amino acids), linked by 18 amino acids from the 5' untranslated region of Nmnat1 [20] [21], [24].
However, evidence for reduced prostate cancer-specific survival was apparent in those cases whose tumor had multiple copies of the fusion.
By comparison, Attard and colleagues (2007) did not present results for overall fusion status however, they did show that fusions caused by deletion had significantly worse disease-specific mortality and this association was largely driven by tumors with two or more copies of the fusion product [ 15].
Furthermore, for the majority of transgenic C. elegans strains the introduced DNA is maintained as an extrachromosomal array, with hundreds of copies of the fusion gene all joined together in each cell, not even integrated into a proper C. elegans chromosome [ 10].
In a hybrid system, display in fusion to pIII commonly results in less than one copy of the fusion protein per phage particle.
The variant T allele of the TMPRSS2 SNP, rs12329760, was positively associated with TMPRSS2-ERG fusion by translocation (p = 0.05) and with multiple copies of the gene fusion (p = 0.03).
