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Multiple ISWpi1 copies occur at various genomic sites in various Wolbachia genomes [19] [21].
The fact that dramatic losses of transgene copies occur in the SOD1 (G93A) line even after a long time of breeding brings one issue to mind that is important in pre-clinical drug studies: Control of copy number.
Such a patchy distribution strongly suggests that horizontal transfers of IS copies occur frequently in Wolbachia.
Second, structural rearrangements that increase the number of EnCYP51 copies occur in individuals carrying the Y136F, which are then favored by further DMI treatments.
Our model does not explain assortment of rDNA given these genes are present in such high copy numbers unless these copies occur in synteny.
This analysis additionally shows that the multiple copies of Hps that are present in OX281 segregate as a genetic unit, indicating that the copies occur together at a single locus.
Similar(52)
Then, if a deletion of the intervening sequence between the two copies occurs followed by a mutation converting the stop codon of the first copy into a sense codon (Fig. 7), this results in the elongation by fusion of the ancestral gene and its copy.
This result suggested that integration or excision of SGI1 copies occurred at the left and/or right side of tandem arrays in the 2nd attB site.
No amplification of 7 pg of spiked plasmid (± 7 × 105 copies) occurred.
Uniparental reductions of homeologous rRNA gene copies occurred in both synthetic and natural populations of Tragopogon allopolyploids.
The most frequently observed che gene to have multiple copies within a che operon is cheY, with multiple copies occurring in 164 operons (Additional file 6).
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