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This means that after intronless retrotransposed copies land onto different genomic locations, the differences between what were formerly the central regions and the boundary regions becomes ambiguous.
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But its first-week sales were only 31,000 copies, landing it at No. 18 on the Billboard chart.
Here, on the quiet S.U.V.-lined street she moved to three years ago, after all, is where news first reached her that "Hell to Raise," which was released last September and has sold 25,000 copies, landed on many critics' top-10 lists for 2005, including those of The New Yorker, National Public Radio and Entertainment Weekly.
When a copy landed on my doorstep to read and review, I couldn't wait to get started and see what happens.
We successfully used SB100x for the generation of transgenic lines harboring one single-copy landing site.
The presence of single copy landing sites was determined by 50% Mendelian segregation of the landing site in an outcross and by Southern blot analysis.
However, as SB100x is a cut and paste transposon (Ivics et al., 1997; Mátés et al., 2009), new single copy landing sites can be readily established by remobilization of single-copy landing sites resulting in local or distant transpositions (Ruf et al., 2011; Yergeau et al., 2011).
To establish randomly placed, single-copy landing sites, we used the well-characterized Sleeping Beauty transposon system 100x (SB100x) (Grabher and Wittbrodt, 2007; Ivics et al., 1997; Mátés et al., 2009).
The ALOG family of proteins encoded by these genes is present in multiple copies in land plants and was claimed to be absent outside of land plants [ 22].
Eminem's hit "Recovery" (Aftermath/Interscope) sold 89,000 copies to land at No. 3, but much of the rest of the Top 10 this week is new.
Also, Nirvana's "Nevermind" (Universal Music Enterprises), reissued last week for the album's 20th anniversary, is No. 13 with 25,000, while a "super deluxe" version, tracked separately, sold 4,000 copies to land at No. 131.
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