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Whereas less than 10 copies in the initial amplification reaction resulted in identification of 15.1% of the samples only, the percentage increased to 54.2% at 10-10 copies, to 95.6% at >10-10 >10-10 and reacopies00% sandles identified foreached Rickettsia species if more than 100%opiesamplespresent identifiedplate.
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k-mers were required to have three copies in the total sequence set, with at least one copy in the initial run and one in the second run.
Sensitivity was calculated for all tick samples as well as tick samples divided into gltA copy numbers in the initial template.
Low copy numbers (≤10) in the initial amplification reaction often resulted in either weak or missing bands in gel electrophoresis with the consequence of insufficient template for subsequent pyrosequencing.
A pBluescript SK vector carrying a Homo sapiens desmin cDNA (accession N° BC032116) was added at 1,000 copies to the initial library in order to assess the normalization efficiency.
To reduce the chances of collecting multiple copies of orthologous proteins in further steps, only clusters that were single copy in this initial step were kept for further screening.
It was firstly down from 8.9 × 10 to 1.7 × 10 copies per nanogram DNA in the initial 20 days (phase I), recovered slightly in phase II, subsequently increased up to reach the highest 3.8 × 10 copies per nanogram DNA in phase III, and finally decreased slightly in last two phases.
To fix the split alleles for the final assembly (Step 5), we examined the position of the two copies in our initial assembly.
There is an alphabet associated with the environment whose elements appear in an arbitrary large number of copies at the initial configuration.
According to a report, Trump told journalists in 1990 that "Trump: the Game" sold some 800,000 copies in its initial run, short of the 2 million copies hoped for by Milton Bradley.
The first volume sold nearly one million copies in the United States in its initial week of release, and each of the two albums (which were also sold as a single package) topped the Billboard album chart.
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