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The gene is carried on the X chromosome, of which women have two copies in each cell.
Ankst started with a low-key cassette release by satirical singer-songwriter (and fellow student) Neil Rosser, who drove the length and breadth of the country, depositing five copies in each of the 12 Welsh-language record shops.
The number of reactive bands in each lane represents the transgene copies in each transgenic line.
SBRS1 is a head-to-tail tandem repeat with tens to hundreds of copies in each region.
Note that the first two copies in each set were used for the case of two video inputs.
It is also a tandem repeat, with usually less than 10 copies in each region, dispersed on all 20 pseudomolecules.
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However, PCR recombinants (chimeras) could still be present among variants which passed the 2-PCRs-3-copies-in-each threshold.
Thus, in the total sample of 159 variants, these 11 PCR chimeras constituted 6.9% of all PEA which passed the 2-PCRs-3-copies-in-each threshold.
We first calculated maximum per amplicon frequency (MPAF) for each putative expressed sequence variant which passed the 2-PCRs-3-copies-in-each threshold.
This heuristic 2-PCRs-3-copies-in-each criterion was useful for revealing general patterns of sequence diversity and relationships, while excluding most sequencing errors.
The consequences of this is illustrated by the fact that from 157 cluster II PEA which passed the preliminary 2-PCRs-3-copies-in-each criterion, 146 (93.0%) were retained after the PCR chimera filtering step.
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