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Specifically, PRSS1 gene copy number in 70 patients had two copies detected using the probe Hs03184214_cn, whereas four patients had only one copy and another patient had five copies (figure 2).
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Similarly, between 100 and 1.000 copies of the ORF73 transcript were detected using the same amount of template that did not reveal any transcriptional activity over Ov9.
Copy number variants were detected using the Control-FREEC software with a 100-kb window size and 20-kb step size.
Multi-copy genes could not be detected using the microarray in this study because their sequence similarity caused cross-hybridization.
RA-CH-1 had four non-core gene families with higher copy numbers, but these were not detected using the KEGG or COG databases.
The greatest number of viral genomic copies was detected using AAV5.
The copy numbers detected using GenoCNA are also shown in Figure 4.
F-test was performed to check whether copy number detected using qPCR showed homogeneity of variance between the reference silkworm and silkworms to be examined.
Compared with increased VEGFA gene copy number, detected using FISH analysis, we identified a significant positive association between increased VEGFA gene copy number (65.9%, 31/47) and increased VEGFA protein expression (75.9%, 41/54) (P = 0.022) (Table 2).
The individual effect of each of our oligonucleotide filters on resolving power is shown is Figure 2 for both human and C. elegans one-copy deletions detected using 50-mer oligonucleotides.
To quantify the growth of F. verticillioides, the copy number of β-tubulin2 (transcriptscript was detected using real-time RT-PCR in seeds collected at 12, 24, 48, 72 and 96 hpi, as reported before.
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