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Genomic portions, having exactly the same profile with regard to size and number as those representing the sampled gene families, were randomly chosen in the S. mansoni genome and the number of TE copies computed (fig. 2).
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The insertion date of the truncated copies was computed as follows: the nucleotide divergence between the truncated copy and the best matching complete copy was computed, and compared to the nucleotide divergence existing between the two LTRs of the complete copy.
The decoded value of the th integer transform coefficient in the th copy is computed as (7).
A gene copy number is computed by counting the number of reads mapped to each known transcript, then computing the median number of copies for each gene over all of its respective transcripts.
In order to provide a deeper characterization of the genotypes, we extended the previously used genetic annotation with significant copy number aberrations computed by GISTIC [34].
Copy numbers were computed for each sample separately using a modified method based on that developed by the Cold Spring Harbor Laboratory [ 28].
In all other eight plant model species, the ERA1 gene is present as a single copy gene, as computed by OrthoMCL (one gene in each species as the orthologous gene, no paralogous genes and no co-orthologous genes).
The occurrences of each family were determined using RepeatMasker (Smit et al. 1996 2010) on the sequenced genomes of An. algerae with the consensus sequences used as library, and the copy numbers were computed using the tool One_code_to_find_them_all (Bailly-Bechet et al. 2014) (supplementary table S2, Supplementary Material online).
The distance to the closest putative gene was then computed for copies present in the same contig as a gene.
The number of copies in Triticeae was computed by Southern blot analysis and slot blotting techniques by using cDNA of Revolver as the probe.
PCR analysis of this region computed a copy number of 4.18+/- 0.45 and 4.47+/-0.58 reinforcing the sensitivity of copy number determinations obtained using the VN approach.
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