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Spoligotyping was performed for all matching strains that had six or more IS 6110 copies by using a commercially available kit, according to the manufacturer's instructions (Isogen Bioscience BV, Maarssen, the Netherlands) (13).
Concentration and DNA quality were measured by using Qubit™ dsDNA HS Assay Kits (Invitrogen Ltd., Paisley, UK) with Qubit® Fluorometer and by gel electrophoresis and converted to the number of copies by using the molecular weight of the DNA.
The PCR sensitivity was previously determined to be ≈100 gene copies by using purified viral DNA, and specificity was determined by SacII restriction enzyme analysis and Southern blot hybridization by using an MaHV1-specific internal probe (5′-GGAGAAGACGTCGCGGTCGTAC-3′) that discriminates MaHV1 from HSV (13 ).
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A month ago, Carlos sold every copy by using the line "Pope says it's O.K. for gay priests to have sex with other gay priests!" "Looks like a story on page A-11," he said, his eyes lighting up.
It achieves low-cost venture copying by using observation and imitation.
In this study, we provide a vector pMoC-eGFP that was efficiently integrated into the sdi1 locus as single copy by using carboxin as selectable agent.
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For example, increasing transposon copy number by using transgenic mice with large transposon concatemers (>30 copies) may lead to local chromosomal rearrangements in subsequent offspring and complicate characterization of causative transposon mutations (Geurts et al. 2006).
Also for the relay nodes, they distribute the duplicate copies to the nodes which do not carry the packet copy yet by using the SIR model of epidemic spreading.
Cycle threshold values were converted to relative copy numbers by using a standard curve of a sample with a known copy number.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com