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The DTSsa5 family has two copies that are highly similar (figure 1A) (< 0.1percentt difference) as a 15 kbp region in the TCR alpha locus in salmon, that contains one of the DTSsa5 copies, appears to have been recently duplicated.
Interestingly, the proportion of methylated and unmethylated copies appears to vary between tissues, even in the case of the intronic RB1 CGI, which in certain adult cell types is biallelically methylated, as judged from methylation levels more than 70% in these tissues (table 4).
In fact, the removal of this massive mid-life proliferation of genomic DNA copies appears to have uncovered a systematic loss of genomic DNA with age (t-test 4 days vs. 19 days, P < 0.0001) (Figs 5d and 6d), to which the loss of tail nuclei we observed via DAPI staining may contribute (Fig. 1).
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In agreement with previous studies of the mouse lemur (genus, Microcebus), the majority of V1Rstrep gene copies appear to be intact and under strong positive selection, particularly within transmembrane regions.
Interestingly, several of these copies appear to be expanded clonally, suggesting that they could be active in B. ovis.
Five of the IS711 copies appear to disrupt genes, which could be a contributing factor to the general process of genome degradation observed in B. ovis.
Two of the remaining copies appeared to be degenerated copies of family III elements, and the other 14 were too degenerated to be unambiguously assigned to one of these families.
However, the short form copies appear to be predominantly translatable.
Four BTB/POZ-ZNF gene copies appear to have been acquired as novel genes in amniotes.
Of the total number of repeats, fifty sequences (926 copies) appeared to be of phage origin.
Nine of the copies appear to be miniature copies shorter than 500 bp (supplementary table S4, Supplementary Material online).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com