Sentence examples for copies and quantitative from inspiring English sources

Moreover, we performed phylogenetic analyses to infer the evolutionary history of the copies, and quantitative PCR (qPCR) to explore whether there is evidence of functional divergence among them.

Dilutions of the plasmid ranged from 10too 300,000 copies per reaction, and quantitative determination for clinical samples was carried out by reading from this standard curve.

To determine whether any parasite DNA could be found to persist in brains of these immunized and challenged mice, PCR with primers to detect the multi-copy B1 gene and quantitative PCR to detect the 300 repeat copy 529 base pair gene was performed with brain from mice infected for prolonged periods of time after immunization with Δrps13 -ATc.

This plea has its origin in issues that were brought to light shortly after the whole human genome sequence was published, followed closely by the genome sequences of most model organisms, and an explosion of discovery relating to single-nucleotide polymorphisms, gene copy-number variation and quantitative trait loci (QTL).

The report begins by documenting this partial chromosomal duplication to identify paralogous gene copies, and then a quantitative analysis of paralogous sequences examines the potential for evolutionary innovation and the importance of the duplication for the genomic repertoire in T. brucei.

Analysis of three copy number calling programs and quantitative PCR showed Birdsuite to have the greatest agreement with quantitative PCR.

While we restrict our analyses to half-sib families, our approach is general and could be applied to any data set with both pedigree and quantitative copy number assay components.

High throughput gene expression analysis by real time PCR in a microfluidic dynamic array was first introduced by Spurgeon et al.[ 40], and has since been successfully applied to copy number variation studies [ 53] and quantitative miRNA expression analysis [ 54].

Hernandez and colleagues [ 61] (2012) identified both qualitative and quantitative differences in copy number between mixed DCIS and matched invasive carcinoma in three of 13 cases (23.1 %) by aCGH.

Based on the sequencing coverage and quantitative PCR results, the copy number of the plasmid is three per genome [ 12].

To investigate the reason why the three FCGR3B probes, located within 500 bp from each other, produced such different results we sequenced the promoter region of FCGR3B and measured the number of gene copies by quantitative PCR (qPCR).