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Multiple alignments of several family copies analyzed by RIPCAL (Hane and Oliver 2008) showed an excess of CpA→TpA and TpG→TpA mutations.
We could not compare ASPM data, as it is present twice (under different Refseq names) in the dataset and both copies analyzed are severely truncated.
The RtPc3-Copia elements clustered in very close branches and showed a high sequence similarity, which could be explained by the low number of copies analyzed and by the fact that intact copies were only identified in P. brasiliensis lineages Pb03 and Pb18.
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For mt genomes with two CR or tRNA copies, we analyzed the first copy.
Concentrations ranging from 10 to 10 copies of analyzed amplicon per reaction were used as the standards for 18S and 10 10 copies per reaction for other genes.
Amplicons of different intragenic copies were analyzed for sequence heterogeneity.
Ct values were inversely proportional to the number of copies of analyzed genes.
From the output subgroups, only those with a minimum of ten copies were analyzed.
In our study EGFR protein expression and number of EGFR gene copies were analyzed by immunohistochemistry and FISH, respectively.
The HRCT images were printed as hard copies and analyzed blindly by two (2003 2004) or three (1996 1997) radiologists.
Aiming for functional similarity of the retrogene and its parental copy, we analyzed only those cases where the parental copy, giving rise to the retroposed gene, had been lost.
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CEO of Professional Science Editing for Scientists @ prosciediting.com