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To further improve the resolution, micrographs with 24 fractions and boxing coordinates were imported into Relion49.
Trimble ® Recon GPSwas used to find the location of each sampling site and the coordinates were imported to GIS platform for preparation of the base map.
At the second study site, also using the 1 m CHM, the Green Hills plot centre coordinates were imported into ENVI 4.2 software and buffered to a distance of 35 m.
The RNP coordinates were imported into Matlab where they were projected onto a vector along the coordinates of the microtubule (Hendricks et al., 2010; Rai et al., 2013), thereby producing on-axis positions of the RNP with respect to the track.
The resulting end-point coordinates were imported to a geographic information system (ArcGIS version 9, Environmental Systems Research Institute), and snapped to the closest points on a linear representation of the river (National Hydrography Dataset, 1 24,000-scale, US Geological Survey, http://nhd.usgs.gov), with manual corrections for errors caused by river bends.
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Raw landmark coordinate data were imported into the computer program MORPHOJ v1.02E [ 60] for shape analysis.
For the student study, the coordinate data were imported into the purpose-built software program of the 3D-PAT, in which the nine postural angles were calculated.
The x, y coordinate values were imported into MS Excel spreadsheets for calculation of the five body angles used for data analysis.
The neuro-fuzzy network estimated the GWQI value for each pixel and then the estimated values with coordinates (X, Y) were imported to ArcGIS environment.
Their 3D coordinates (x, y, z) were imported into Exce using the Immersion software package (Immersion, Inc., San Jose, CA, USA).
Twenty-one morphological landmarks were plotted on each photograph (Fig. S1); the coordinates of those landmarks were imported into the software TPSRELW (Rohlf 2006), which was used to identify diversity of shape variation within and between the crosses and treatments, independent of body size.
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