The latter was done using specific plots δn via erf(x/d), where x is the coordinate measured from the glass surface to the bulk, d is the characteristic diffusion depth, and δn is the difference between normalized index variation profile and the closest erfc-function.
Where x is the longitude coordinate, z the coordinate measured from the mid-plane of the beam and t is time.
A blunt 1-μl Hamilton syringe angled at 10° (with the tip pointing toward the rat's tail) was aimed at the following coordinates relative to bregma: anterior-posterior – 4.5 mm, medial-lateral ± 1.0 mm, dorsal-ventral (DV) −9.2 mm (DV coordinate measured from dura).
For experimental spatiotemporal datasets, spatial information is often recorded as a set of pixel coordinates measured directly from the microscopic images, whereas time is usually considered as a sequence of regular time frames.
2) The coordinates were measured from each still image using the coordinate-measuring tool in ImageJ (US National Institutes of Health, Bethesda, MD).
All dorsal ventral coordinates were taken from dura, and anterior posterior coordinates were measured from bregma.
AP and ML coordinates were measured relative to bregma, whereas DV coordinates were measured from the brain surface.
The rostrocaudal distance of each cell body or process was measured from the section number, and the dorsoventral and mediolateral coordinates measured using Corel graphics programs.
The following coordinates (mm, measured from bregma) were used to locate the OPFC: site (i): AP +3.7, L ±1.2, DV −4.8; site (ii): AP +3.7, L ±2.8, DV −4.4.
Samples (brain homogenates and PBS) were injected into the right hippocampus using the following coordinates as measured from bregma: antero-posterior (AP), -1.8 mm; medio-lateral (ML), -1.8 mm; dorso-ventral (DV), -1.8 mm.
