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Processed data files (density maps, wig format; peak coordinates, bed format) were acquired from the NCBI Gene Expression Omnibus (GEO) and Short Sequence Read Archive SRAA).
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Tag coordinate bed files of RNAP II in human CD4+ T cells using the Solexa sequencing technology were also used [ 34].
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Files with peak coordinates in bed format were downloaded from the NCBI GEO depository [GEO GSE38379].
To analyze multiple loci, users may specify the input genomic coordinates in BED format, which is widely used in high-throughput genome analyses.
For a consistent comparative analysis, the tool LiftOver was used to convert genomic coordinates in BED format from human to canine, using the UCSC [ 13] chain file for hg19toCanFam3 as reference.
The output delivered to the enduser consists of BED coordinates for all the HMM matches found in the sequence along with the final count of non-overlapping HMM matches and their classification by size.
Patterns of conservation within a target sequence can be aggregated using the halLiftover tool, which maps coordinates in a BED file to an arbitrary target in the alignment.
The coordinates in the BED file provided by Nam and co-workers were lifted from the mm9 to mm10 mouse genome using the LiftOver tool from UCSC genome browser (http://genome.ucsc.edu/cgi-bin/hgLiftOver).
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