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Hydrodynamic characterization and in vivo coimmunoprecipitation results suggested that the HsGEN1 fragment is a monomer in solution and oligomerizes on DNA to conduct a coordinated cleavage of four-way junctions (Rass et al. 2010).
These observations demonstrate the highly specialized role of these enzymes for the dual, coordinated cleavage of target substrates, unlike the single incision event that occurs for flap endonucleases such as XPF ERCC1 or XPG (Guzder et al. 1995; Aboussekhra et al. 1995).
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The non-conventional splicing of XBP1 mRNA requires coordinated cleavage and ligation events.
It was envisioned that the coordinated, symmetrical cleavage of HJs across one of two alternative planes, as indicated in Fig. 2, could provide the mechanistic basis for the formation of crossover (CO) and non-crossover (NCO) recombinants.
The structure-specific nucleotide excision repair nucleases XPG and ERCC1-XPF recognize DNA substrates that contain single-stranded DNA double-stranded DNA double-stranded as bubble substrates anDNAairpins, to initiate repair through coordinated 5′ and 3′ cleavage of the lesion by ERCC1-XPF and XPG, respectively.
Challand et al. observed the addition of glyoxylate to assays limits Cα Cβ tyrosine bond cleavage, suggesting dehydroglycine might modulate uncoupled cleavage of SAM as a mechanism ensuring dehydroglycine production and incorporation into the thiazole carboxylate is a coordinated event between ThiH and ThiG.
(A D) Cleavage of ovum.
Left, thrombin cleavage of T4L-LVPRGS-PL5.
Cleavage of SNX2 disrupts interaction with Vps35.
Here the cleavage of PTPRS is illustrated.
Right, Ni2+ cleavage of T4L-GSHHW-PL5.
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