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We find in both cases that spike patterns generated by independent coding and coordinated assembly models can be distinguished by the shuffling analysis and by the prediction analysis.
The additional model includes interactions between cells within the population in order to simulate data under the coordinated assembly hypothesis, while the additional simulations compare the behavior of the independent coding and coordinated assembly models when subjected to statistical tests of independence.
To address these questions, we analyzed experimental data along with data generated by independent coding and coordinated assembly models, using a modified version of this shuffling procedure (see 'Materials and methods').
Presynaptic development and function relies on coordinated assembly, transport and delivery of synaptic vesicle precursors, pre-assembled synaptic cytomatrix proteins and mitochondria (Goldstein et al., 2008; Maday et al., 2014; Maeder et al., 2014).
In contrast, the coordinated assembly hypothesis predicts that groups of cells show additional coordination beyond that imposed by a fixed firing rate and phase code (Harris et al., 2003; Harris, 2005).
Below, we investigate some functional consequences of the independent coding and coordinated assembly hypotheses and show that, despite the advantage of assembly coordination in generating robust sequential activity patterns, it suffers from severe limitations in remapping and storage of multiple spatial maps.
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To provide benchmarks for the interpretation of experimental results, we also analyzed simulated datasets generated with either independent coding or coordinated assemblies.
Therefore, based on comparison of simulated with experimental datasets, coordinated assemblies appear unlikely to account for the observed activity in CA1.
In the Discussion we outline which previous models of phase precession could provide a mechanistic basis for our single cell coding model and which models would instead imply coordinated assemblies.
How HSV1 coordinates assembly with transport remains an important unresolved question.
d4EHP binds the 5' cap of cad mRNA, while Bcd binds the 3'UTR, preventing the coordinate assembly of the translational machinery [13].
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