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However, Model 2 is still incomplete as it does not capture the reverse or symmetrical cooperative dependence, when one Ca2+ binding site cooperatively influences another, and another site can in the same way influence the first site, within both terminals.
Model 2 is an intermediate model which extends the previous representation by introducing the cooperative dependence of one site on another in each domain.
When substrate is limiting, we observe cooperative dependence on histone H3 with p300, but not CBP, while the dependence on H3/H4 displays cooperativity with CBP but not p300.
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Most surprisingly, the lipid extracting effect has an all-or-none cooperative concentration dependence that is similarly to a MIC.
Since the characteristics such as cooperative competition, professional dependence, compromise, overall utility and so on exist in multidisciplinary collaborative design (MCD), an effective way to gradually eliminate the conflicts among the multiple disciplines and reach an agreement is the negotiation by which a compromise solution that satisfies all parties is got.
The binding of glycine betaine to purified OpuA and OpuAC (KD = 4 6 µM) did not show any salt dependence or cooperative effects, in contrast to the transport activity.
An apparent extension of this work is to consider the dependences among cooperative domains in multidomain proteins.
The apparent dissociation constants (K D) of CGP 12177 and propranolol for this secondary (allosteric) conformation could be determined from the concentration dependence of the cooperative effect of each ligand on the BODIPY-TMR-CGP dissociation rates.
Despite its large size and modular architecture (15 repeats), YopM equilibrium unfolding is highly cooperative, and shows a very strong dependence on the concentration of urea.
To predict the yield behavior, we extended the modified cooperative model with temperature and strain rate dependences for neat polymers by incorporating a three-phase approach and a two-population model.
The observed sigmoidal dependence of Kb on X confirms the cooperative character of the binding.
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