The present study evaluated the interactions among pre-cooling, cryoprotectant, cooling, and thawing for rhesus monkey sperm using a four-way factorial design.
Our findings showed strong interactions among cryoprotectants, cooling, and thawing rates, but not pre-cooling rate, on post-thaw motility and forward progression.
The loops were sampled at euthanasia by cutting the mesentery and immediately cooled on thawing ice.
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In 2011, in an attempt to show the Western world that the Myanmar government was cool, Thaw and hundreds of other dissidents were released early.
The optimal combination of cooling and thawing for maximum post-thaw survival depended on the types of cryoprotectant.
Because variable cooling and warming rates have been observed with some methods[10], our effort began by defining reproducible processes for cryopreserving and thawing mouse sperm.
Spermatozoa were subjected to controlled cooling and thawing in MiniFlatPacks (MFPs) and examined for motility (using computer-assisted sperm analysis, CASA) at selected stages of processing.
Challenges include difficulty to achieve extreme cooling and thawing rates, toxicity of high cryoprotectant agent (CPA) concentrations needed to vitrify suspensions at a realizable temperature, devitrification upon rewarming, and inability to accommodate freezing of large volumes of blood using microliter cryo vials.
These were transported to the Slovak Medical University in Bratislava, in thermo boxes with cooling cartridges to prevent thawing, and stored at −18°C.
These were transported to the Slovak Medical University in Bratislava in thermo boxes with cooling cartridges to prevent thawing, and stored at −18°C for PCB analysis; a small amount (0.2 mL) was aliquoted for lipid measurement and stored.
