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Reactions in such dynamic rapidly cooling reaction systems with a meteoritic composition have not been intensively studied before, and final products in such reaction systems have not been known either.
During cooling, reaction kinetics become sluggish, and metastable mineral assemblages and compositions can be preserved well outside their normal stability fields.
The cooling reaction mixture was diluted with ice water (30 mL), extracted with ethyl acetate (30 mL × 3).
After brief cooling, reaction mixture was injected into the preparative HPLC system consisting of Luna C18 (2) reversed phase column (Phenomenex, Torrance, CA, USA).
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To the cooled reaction mixture, 1 mL of water was added, and the resulting mixture was loaded into the HPLC loop.
The cooled reaction mixture was poured into water (3 mL/mmol) and extracted with ethyl acetate (3×3 mL/mmol).
The cooled reaction mixture was treated dropwise with 1 M HCl (24 mL).
For a maximum yield it is customary to add the ether solution slowly, under vigorous stirring of the cooled reaction mixture.
To the cooled reaction mixture was added 41.0 μL (51.0 mg, 0.23 mmol) of TMSOTf, and the reaction mixture was stirred at 0 °C for 15 min, at which time it was poured into a mixture of 20 mL of ethyl acetate and 20 mL of satd aq NaHCO3.
The reverse transcription was then performed with the Superscript III kit (Invitrogen), by adding 4 µL 5x First strand buffer, 40 U DTT, and 200 U Superscript III to the cooled reactions.
In addition, after reaction and cooling the reaction system, benzenesulfonic acid grows solid.
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