Sentence examples for cooling interval from inspiring English sources

For determination of bacterial GSH content, bacteria were collected after 24 h of cultivation and disrupted by sonication (Bransonic 3200, Branson Ultrasonics b.v., Soest, the Netherlands) on ice for 10 min with 3 sec cooling interval per min. Suspensions were centrifuged, yielding a cell-free extract.

E. coli cells were lysed on ice by 10 15 repeats of 10 s of sonication (Heidolph Instruments) with a 20 s cooling interval and then centrifuged (26000  g, 1 h, 4°C).

Cells were incubated in lysis buffer (7M Urea, 2M thiourea, 2% (w/v) CHAPS) for 30 min on ice and sonicated five times in an ultrasonic water bath, where each sonication was performed for 10 s followed by 10 s cooling interval on ice.

The mixture was sonicated on ice (eight 10 s pulses with 20 s cooling intervals) by using a 1/8-inch sonication probe (FB-50, Thermo Fisher Scientific).

Each sample was sonicated 6 times in an ice-cold water bath for 15 s each, with cooling intervals of 1 min 45 s in between.

The cells were lysed by sonication (six 30 seconds bursts, with 30 second cooling intervals on ice at an amplitude of 65%) and insoluble debris removed by centrifugation at 37, 000×g for 15 minutes.

After the addition of 300 mg of glass beads (∼100 µm diameter), cells were lysed by three rounds of bead beating in a Fastprep machine at force 6.5 for 30 s with cooling intervals of 5 min on ice in between.

They typically consisted of 10 repeats of 30 pulses separated by 30 s cooling intervals with an output setting of 4 and a duty setting of 40.

M. smegmatis cells (10 g wet weight) were washed and re-suspended in 30 ml of buffer A, containing 50 mM MOPS (adjusted to pH 8.0 with KOH), 5 mM β-mercaptoethanol and 10 mM MgCl2 at 4 °C and subjected to probe sonication (Soniprep 150, MSE Sanyo Gallenkamp, Crawley, Sussex, UK; 1 cm probe) for a total time of 10 min in 60 s pulses and 90 s cooling intervals between pulses.

The 100 g of yeast cells "popcorn" [50] were disrupted in Freezer Mill 6870 (SPEX Sample Prep LLC, NJ, U.S.A). in liquid nitrogen by seven disruption cycles (2 min each at 15 pulses/sec with 2 min cooling intervals).

After the addition of phenylmethylsulfonylfluoride (PMSF; 0.5 mM) and mammalian protease inhibitor cocktail (1 mM AEBSF, 0.08 μM aprotinin, 20 μM leupeptin, 40 μM bestatin, 15 μM pepstatin A and 14 μM E-64; Sigma Chemical Co., St . Louis MO), tissue powder was homogenized on ice using a Brinkmann PT3000 polytron with 10 second bursts and 30 second cooling intervals.