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Then 1 mL of cool filtrate was mixed with 5 mL distilled water and a few drops (2-3) of 10% ferric chloride were added.
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The neutralized cooled filtrate was extracted with dichloromethane.
The mixture was filtered while hot and the cooled filtrate made alkaline to litmus paper with 20% sodium hydroxide solution.
Extracts were cooled, filtrated and preserved until element analysis.
The mixture was warmed to 100°C and stirred for 1 h; then it was cooled, filtrated, and dried in vacuum oven for overnight.
After cooling, the filtrate was acidified to pH 1 to form a precipitate which was collected by filtration and dried to give 0.2 g (30%).
After cooling the filtrate was shacked with equal volume of chloroform and lower layer was separated and shacked with dilute ammonia.
After homogenization, the mixture was boiled in 5 min, cooled and filtrated.
After mixing, the mixture was boiled for 5 min, cooled and filtrated.
After cooling of the filtrate and evaporation of solvent, the pure crystals of products were obtained.
The filtrate was cooled to room temperature and used as reducing agent and stabilizer.
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