Images were taken with the fluorescence microscope MD5000B (Leica, Wetzlar, Germany), equipped with a cool charge-coupled device camera ORCA-ER (Hamamatsu Photonics, Hamamatsu, Japan) and iVision-Mac software (BioVision Technologies, Exton, PA).
The instrument is equipped with Peltier cooled charge coupled device (CCD 576x400 pixels).
The scattering signal was collected confocally and detected with an integrated Peltier cooled charge coupled device (CCD) camera.
The resulting PL was detected by a liquid nitrogen cooled charge coupled device after passing through a grating monochromator.
Raman spectra were recorded on a LabRam HR800UV Raman spectrometer (Horiba Jobin-Yvon) attached with a confocal microscope and liquid nitrogen cooled charge coupled device detector.
BLI encompasses non-invasive imaging of luciferase gene activity using cooled charge coupled device cameras in luciferase transgenic animals or in grafted, luciferase transgenic cells.
The emission signal is magnified 1.6 <img src="http://journals.plos.org/plosone/article/asset?id=info?doi/10.1371/journal.pone.0016890.e336.PNG" class= inline-graphic"/> and detected by a back-illuminated, thermoelectric cooled charge coupled device (EMCCD) detector (iXon) (Andor, Ireland).
Full-frame images were captured with an Evolve® cooled charge coupled device camera (Photometrics, Tucson, AZ) at 30 frames/sec (fps) using EasyRatioPro software (Photon Technologies Int'l).
Fluorescent images were captured using a cooled charge coupled device camera (Princeton Scientific Instruments, Trenton, NJ) or a Zeiss LSM 510 META laser-scanning microscope (Carl Zeiss MicroImaging, Inc., Thornwood, NY).
Images of metaphase spreads were captured using a Nikon® E800 epifluorescent microscope equipped with a Ludl 6 position filter wheel and a Photometrics Sensys™ cooled charge coupled device (CCD) camera.
Specific targeting to the B-cells was demonstrated in the cell mixtures by use of fluorescent microscopy combined with a sensitive technique to detect low levels of AlSPc fluorescence, a cooled charge couple device (CCD) camera.
