Sentence examples for converted to single from inspiring English sources

Total RNA was extracted from each cell line using TRIzol Reagent (Invitrogen), and converted to single stranded cDNA using Superscrip III (Invitrogen).

For amplification-based sequencing, messenger RNA (2 µg) was fragmented at 85°C for 5 min in a fragmentation buffer (Ambion) and converted to single stranded cDNA using SuperScript II reverse transcriptase (Invitrogen), followed by second-strand cDNA synthesis using Escherichia coli DNA polymerase I (Invitrogen).

The sets of acquired high-resolution MS and MS/MS spectra for peptides were converted to single data files and they were merged into Mascot generic format files for database searching.

Pollutant maps prepared from spatial interpolation were converted to Single Factor Pollution Index (SFPI) maps using Eq. 6: P_{ijk} = frac{{C_{ijk} }}{{S_{j} }} (7 where (P_{ijk}) is the SFPI value and (C_{ijk}) is the measured concentration at the ith location for the jth water pollutant of the kth year.

All MS2 spectra were converted to single DTA files using Bioworks 3.3 at default settings.

Subsequently, 1 µg of total RNA was converted to single strand cDNA using Quantiscript Reverse Transcriptase (Qiagen).

"If those buildings were renovated and converted to single-family, they could go for in excess of $25 million each," Mr. Miller said.

H OUSES converted to single-family status by previous owners or ones that can be emptied by the sellers are worth significantly more than those with tenants in residence.

The training set was converted to single-label and multi-label training sets.

Once converted to single-use systems, botanical resources are lost as natural vegetation is cleared and the land is drained for agriculture.

Table 15 shows the first-year replacement cost of the seven case study wetlands if converted to single-use agricultural systems.