Toca 511 (vocimagene amiretrorepvec) is an investigational nonlytic, retroviral replicating vector (RRV) that delivers a yeast cytosine deaminase, which converts subsequently administered courses of the investigational prodrug Toca FC (extended-release 5-fluorocytosine) into the antimetabolite 5-fluorouracil.
DNase treated (Ambion, Texas, US) total RNA was converted subsequently to cDNA using random hexamers and Superscript III (Life Technologies) according to standard procedures.
Once the dynabead-polyadenylated RNA binding was reversed chemically, the polyadenylated RNA was used as a template for first-strand synthesis that was converted subsequently to double-stranded cDNA.
He was not Christian, but was soon converted and subsequently married Eormenhilda, daughter of Eorcenberht of Kent and Seaxburh.
PEP is also converted to pyruvate which is subsequently converted to acetyl-CoA and formate by pyruvate formate lyase (PFL), and to lactate by lactate dehydrogenase (LDH).
Glutaminase converts glutamine to glutamate which is subsequently converted to α-KG by glutamate dehydrogenase [ 50].
The remainder is converted to fluorouridine monophosphate, which is subsequently converted to fluorouridine diphosphate and then to either fluorouridine triphosphate (FUTP) or fluorodeoxyuridine monophosphate (FdUMP) (Schilsky, 1992).
Procaspase-1 is converted to activated caspase-1, which subsequently converts interleukins 1beta (IL-1β) and IL-18 into their active secreted forms [ 5- 7].
In PAT, light is absorbed by biological tissue and converted to transient heating, which is subsequently converted into an ultrasonic wave due to thermoelastic expansion.
Manganese superoxide dismutase (MnSOD) is the primary mitochondrial ROS scavenging enzyme that converts superoxide to hydrogen peroxide, which is subsequently converted to water by catalase and other peroxidases.
