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To control for the absence of over-conversion, we methylated the CpGs of the DNA in vitro with SssI methyltransferase and then subjected it to bisulphite conversion in parallel with the test reactions and the protocol described above.
Similar(59)
LC3 conversion assays performed in parallel with C57BL/6 and C57BL/6 lpr/lpr T cells showed no significant difference between both strains even at the highest ages tested for comparison of MRL lpr/lpr and CBA/J (Fig. S2 ).
A 4-methylumbelliferone standard curve ranging from 5 nM to 25 μM was run in parallel for conversion of fluorescence data to AGAL activity expressed as nmol/mg protein per hour.
JAERI is conducting R&D on the power conversion system of the GTHTR300 plant, in parallel with plant design work.
Although high-throughput SNP genotyping platform such as GoldenGate assay of Illumina are available that allows genotyping of large number of SNPs (e.g. 1,536) in parallel [ 60], conversion of SNPs into CAPS assay is a cost effective method that can be used in low-tech laboratories [ 61].
This result also indicates that the accumulation of apiforol and luteoforol is influenced by a flavonoid 3'-hydroxylase acting in parallel for the conversion of naringenin to eriodictyol.
To realize a serial-to-parallel conversion in this experiment, we used a sum-frequency generation nonlinear crystal (SFG-NLC) and a four-bit ultrashort pulse sequence as a time-gating device and a gate pulse sequence, respectively.
A control experiment at Drosophila melanogaster DNA, performed in parallel, resulted in a conversion of 169 out of 170 cytosines to thymine.
In parallelizing, we assert these in parallel.
The first proposed converter is based on mixed radix conversion (MRC) considering two moduli at a time in parallel and combining the residues using MRC.
In parallel, we also performed Resorufin conversion assay to measure cytochromeP450 enzymatic activity in liver tissues.
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