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Such a characterization is important given that lamina X represents a unique sexually dimorphic region that is a convergence site for somatic and visceral afferent inputs, which includes nociceptive information.
The second project I referred to (above if you skipped down, which I hope you didn't) would be the convergence site that my fellow senior classmates and I worked on for the better part of a year.
Both the size and serotonin levels of synaptic puncta (Figures 8f, 8g) were higher at the convergence site that elsewhere.
This is a known convergence site for visual articulation and auditory features, and has been shown to depend on the comodulation of audiovisual stimuli [87].
However, in order to better identify the upstream convergence site, we tested the hypothesis that the NMDA receptor itself serves as a coincident-detector of NMDA and dopamine neurotransmission.
We also found sites in the Substantia Nigra containing higher numbers of converging dendrites, associated with clusters of puncta expressing synaptophysin (Figures. 8c, 8d) at a higher level than those found beyond the convergence site (Figure 8e).
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These convergence sites, termed dendro-dendritic contact clusters (DCCs), were first characterized in terms of dendritic behavior.
Using such values for dendro-dendritic contacts in 3D, we detected areas within the substantia nigra containing up to 8 convergence sites (equals to 3.2×105 per mm3), each including 3 6 dendritic branches (Figures. 8a, 8b).
At hubs of convergence sites, the incidence of axo-dendritic contacts as well as clustering levels, pre- and post-synaptic protein content and secretion capacity of synaptic connections are higher than found elsewhere.
In this report, we find that dendritic branches of different dendritic trees converge in an activity-promoted fashion, resulting in clustering and strengthening of synaptic connections at the convergence sites.
Thus, future clinical trials must be carefully designed to identify the key convergence sites and to employ appropriate therapies designed to adequately impinge on their downstream effectors.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com