Sentence examples for conventional restriction from inspiring English sources

To date, genetic manipulation of A. pullulans mainly relies on time-consuming conventional restriction enzyme digestion and ligation methods.

The objective of the paper is to overcome the conventional restriction to the field of rational functions in the algebraic design of control systems.

This assay is reliable, economical, and can be performed by less trained technologists compared with the procedure performed by the conventional restriction fragment length polymorphism technique.

The assay was validated by the comparison of the genotyping results of 50 blood specimens from the LightCycler polymerase chain reaction with the conventional restriction fragment length polymorphism procedures.

A modulation angle parameter unique to the complex representation gives an additional degree of freedom and can be used to model the basic shape of the pulse, thus lifting the conventional restriction to fixed pulse shapes in real-valued correlation techniques.

The eGFP-pSV40-NeoR reporter cassette was obtained by conventional restriction cloning.

But the Surrealistic impulse is still very much alive in contemporary art, and there's a good reason: whether or not it reveals mystic truths, the free play with disjunctive, contradictory and paradoxical images, materials and forms has a way of relaxing conventional restrictions on creative imagination.

More similar to the conventional restriction-ligation system, compatible cohesive ends can be generated in alternative ways.

Consequently, the developed method is most suitable to use when no appropriate pair of Type II restriction endonucleases for the conventional restriction-ligation strategy is at hand or available.

It should be noted that with two internal SacI and one internal KpnI recognition sites, this DNA fragment cannot be cloned accordingly into the multiple cloning site of the vector using the conventional restriction-ligation strategy.

Consequently, the influence on the process of interventions based on conventional restrictions of antibiotic use or hosts' isolation procedures might not be successful beyond the red line, forcing us to explore the possibilities of other type of interventions (Baquero et al. 2011; Laxminarayan et al. 2013; Fowler et al. 2014) necessarily applied within the frame of global ecological improvements.